adipogenesis marker antibody sampler kit  (Cell Signaling Technology Inc)

Journal: eLife

Article Title: Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function

doi: 10.7554/elife.84168

Figure Lengend Snippet: Figure 3. MTIF3 perturbation in mature adipocytes does not affect adipocyte-specific protein expression or total triglyceride content. (A) An illustration of Cas9-specific single guide RNA (sgRNA)-binding site in the exon expressed in all three MTIF3 protein-encoding transcripts. (B) Representative Sanger sequencing of control and knockout hWAs mature adipocytes. (C) Immunoblots of adipocyte markers in scrambled control and MTIF3 knockout adipocytes, n = 5 independent experiments. (D) Quantitative analysis of MTIF3 band densities in (C). (E) Quantitative analysis of ACC, FABP4, and FAS band densities in (C). (F) Representative Oil-red O staining images of control and MTIF3 knockout in hWAs mature adipocytes. Scale bar is 200 µm. (G) Total triglyceride content in scrambled control (SC) and MTIF3 knockout (KO) cells. n = 3 independent experiments. Error bars show standard deviation in all plots. Statistical analysis was performed using two-tailed Student’s t-test, p values are presented in each graph. Uncropped blot images for (C) and raw.scn data files can be found in Figure 3—source data 1.

Article Snippet: After blocking in 5% bovine serum albumin (BSA) solution for 1 hr, the membranes were incubated with primary antibodies against MTIF3 (14219- 1- AP, Proteintech), OXPHOS complex (45- 8199, Thermo Fisher Scientific), FABP4, ACC, FAS (12589, Cell Signalling Technology), ATP8 (26723- 1- AP, Proteintech), ND2 (19704- 1- AP, Proteintech), CYTB (55090- 1- AP, Proteintech), and corresponding horseradish peroxidase (HRP)- conjugated secondary antibodies (anti- mouse IgG, Cell Signalling Technology; anti- rabbit IgG, Cell Signaling Technology).

Techniques: Expressing, Binding Assay, Sequencing, Control, Knock-Out, Western Blot, Staining, Standard Deviation, Two Tailed Test

Journal: eLife

Article Title: Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function

doi: 10.7554/eLife.84168

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-FABP4 (rabbit polyclonal antibody) , Cell Signalling Technology , Cat: 12589 , WB (1:1000).

Techniques: Recombinant, Plasmid Preparation, Sequencing, TaqMan Assay, DNA Extraction, Isolation, Sample Prep, Picogreen Assay

Journal: eLife

Article Title: Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function

doi: 10.7554/elife.84168

Figure Lengend Snippet: Figure 3. MTIF3 perturbation in mature adipocytes does not affect adipocyte-specific protein expression or total triglyceride content. (A) An illustration of Cas9-specific single guide RNA (sgRNA)-binding site in the exon expressed in all three MTIF3 protein-encoding transcripts. (B) Representative Sanger sequencing of control and knockout hWAs mature adipocytes. (C) Immunoblots of adipocyte markers in scrambled control and MTIF3 knockout adipocytes, n = 5 independent experiments. (D) Quantitative analysis of MTIF3 band densities in (C). (E) Quantitative analysis of ACC, FABP4, and FAS band densities in (C). (F) Representative Oil-red O staining images of control and MTIF3 knockout in hWAs mature adipocytes. Scale bar is 200 µm. (G) Total triglyceride content in scrambled control (SC) and MTIF3 knockout (KO) cells. n = 3 independent experiments. Error bars show standard deviation in all plots. Statistical analysis was performed using two-tailed Student’s t-test, p values are presented in each graph. Uncropped blot images for (C) and raw.scn data files can be found in Figure 3—source data 1.

Article Snippet: After blocking in 5% bovine serum albumin (BSA) solution for 1 hr, the membranes were incubated with primary antibodies against MTIF3 (14219- 1- AP, Proteintech), OXPHOS complex (45- 8199, Thermo Fisher Scientific), FABP4, ACC, FAS (12589, Cell Signalling Technology), ATP8 (26723- 1- AP, Proteintech), ND2 (19704- 1- AP, Proteintech), CYTB (55090- 1- AP, Proteintech), and corresponding horseradish peroxidase (HRP)- conjugated secondary antibodies (anti- mouse IgG, Cell Signalling Technology; anti- rabbit IgG, Cell Signaling Technology).

Techniques: Expressing, Binding Assay, Sequencing, Control, Knock-Out, Western Blot, Staining, Standard Deviation, Two Tailed Test